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. 2023 Feb 9;13:1106315. doi: 10.3389/fcimb.2023.1106315

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Copyright © 2023 Jones, Mangin, Reynolds, Villanueva, Cruz, Versteeg, Keegan, Kendricks, Pollet, Gusovsky, Bottazzi and Hotez

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Antigen specific TH1/TH2/TH17 cytokine release from splenocytes. Splenocytes were restimulated in vitro with recombinant Tc24-C4 protein or cRPMI alone (unstimulated) as described. Culture supernatants were harvested after 96 hours and stored at -80C until quantification of cytokines by Luminex assay. Data was analyzed for normality, then treatment groups were compared to controls using parametric or non-parametric ANOVA depending on normality. When comparing groups to the infected untreated control *, p≤0.05; **, p≤0.01; ***, p≤0.001; ****, p≤0.0001. When comparing groups to E6020 only treatment $, p≤0.05. $$, p≤0.01; $$$, p≤0.001; $$$$, p≤0.0001.