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. 2023 Feb 9;13:1106315. doi: 10.3389/fcimb.2023.1106315

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Copyright © 2023 Jones, Mangin, Reynolds, Villanueva, Cruz, Versteeg, Keegan, Kendricks, Pollet, Gusovsky, Bottazzi and Hotez

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Antigen specific cytokine producing T cells. Splenocytes were restimulated in vitro with recombinant Tc24-C4 protein or cRPMI alone (unstimulated), then stained with fluorescent antibodies for analysis by flow cytometry as described. Results are expressed as a ratio of antigen specific cells divided by unstimulated cells. (A) CD4+IFNγ+ cells, (B) CD8+IFNγ+ cells, (C) CD4+IL-4+ cells, (D) CD8+IL-4+ cells. When comparing groups to the infected untreated control *, p≤0.05; **, p≤0.01; ***, p≤0.001.