Figure 11.

Progression of 4T1 tumor spheres on the Gel/SA/dECM scaffolds. (A) Confocal laser microscopy was used to observe the progression of tumor spheres on the three groups of scaffolds for 1 d, 4 d, and 7 d (scale bar: 200 μm). (B) Growth and morphology of 4T1 in 2D environment (scale bar: 200 μm). (a), (b), (c), and (d) show the images of 4T1 under optical microscope on 1 d, 3 d, 5 d, and 7 d, respectively. (C) Quantitative detection of the change of tumor sphere diameter on the scaffolds on 3 d, 5 d, and 7 d. The diameters were evaluated by IPP software. *P<0.05, **P<0.01 represent significant difference between two groups.