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. 2022 Oct 28;9(1):630. doi: 10.18063/ijb.v9i1.630

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Copyright: © 2022, XU, et al.

This is an Open-Access article distributed under the terms of the Creative Commons Attribution-Noncommercial License, permitting all noncommercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Cell viability, distribution, and growth of 4T1 cells in the Gel/SA/dECM hybrid scaffolds and 2D culture condition were both investigated. Fluorescence staining was performed on the scaffolds and cells at 1 d (A), 4 d (B), and 7 d (C), and the growth of 4T1 on the scaffold was observed with confocal laser microscope. Green: live; red: dead; white arrows: cell colonies or tumor spheres (scale bars: 500 μm). (D) Viability of 4T1 grown on scaffolds evaluated by CCK-8 kit. *P<0.05, **P<0.01 represent significant difference between two groups.