Fig. 2.

TEADs interacts with DNA repair proteins. A, bar graph representing the abundance (average sum PSMs) of canonical binding partners, YAP, TAZ, and VGLL4 binding to TEAD equally in HEK293 cells. B, as described in (A) but for Detroit 562 cells. C, co-immunoprecipitation assay comparing MYC-tagged WT TEAD3 and MYC-tagged NLS mutant (NLSm) TEAD3. Figure is representative of three or more replicates. WCL, whole cell lysate. D, co-immunoprecipitation of MYC-tagged WT TEAD3 after treatment with 20 μg/ml bleomycin (BLM) for the indicated time points. Figure is representative of three or more replicates. ∗indicates a nonspecific band detected along with RIF1. RIF1, Rap1-interacting factor 1; RIF1, Rap1-interacting factor 1; TEADs, TEA domain family members 1 to 4; TAZ, transcriptional co-activator with PDZ-binding motif; PARP1, poly(ADP-ribose) polymerase 1; XRCC6, X-ray repair cross-complementing protein 6; XRCC5, X-ray repair cross-complementing protein 5; YAP, Yes-associated protein.