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. 2023 Jan 12;22(2):100496. doi: 10.1016/j.mcpro.2023.100496

Fig. 5.

Fig. 5

Depletion of TEADs by RNAi decreases NHEJ and HR DNA repair in response to double-stranded breaks. A, schematic of the NHEJ GFP-reporter assay in HeLa cells. B, depletion of TEAD1-4 by siRNA significantly decreased NHEJ repair efficiency. Positive controls for NHEJ are siLIG4 and si53BP1. Three biological replicates were performed, and at least 10,000 cells were analyzed per condition. Statistical analysis was performed using GraphPad Prism software, ∗∗p < 0.01. C, schematic of the direct-repeat (DR) GFP-reporter assay for HR in U-2 OS cells. D, depletion of TEAD1-4 by siRNA significantly decreased recombination repair efficiency. The positive control for HR is siBRCA2. Three independent experiments and three technical replicates in each experiment were performed. In all cases, 10,000 cells/well were plated and cells were analyzed using an Attune N × T Flow cytometer after 48 h. Statistical analysis was performed using GraphPad Prism software, ∗∗∗∗p < 0.0001. HR, homologous recombination; NHEJ, nonhomologous end joining; TEADs, TEA domain family members 1 to 4.