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. 2023 Feb 6;60:102628. doi: 10.1016/j.redox.2023.102628

Fig. 5.

Fig. 5

MARS2 regulates EMT via redox regulation. a. Transcriptional expressions of EMT markers (E-cadherin, Slug, Snail and Twist) were evaluated by qRT-PCR upon MARS2 knockdown in A549 cells. si-Cont indicates si-control RNA (n = 9). b. Morphological change of TGF-β-induced A549 cells upon MARS2 knockdown (n = 3). c. Confocal microscopic image of E-cadherin level in A549 cells upon MARS2 knockdown (n = 3). d. Cellular E-cadherin level was checked by western blot analysis upon MARS2 knockdown, and a rescue assay was performed with exogenous MARS2 expression in A549 cells (n = 3). e. Transcriptional expression of E-cadherin was evaluated by qRT-PCR upon MARS2 knockdown, and a rescue assay was performed with exogenous MARS2 expression in A549 cells (n = 9). f. qRT-PCR of transcriptional expressions of p53 and E-cadherin in A549 cells upon MARS2 knockdown, TP53 knockdown and double knockdowns of MARS2 and TP53 (n = 9). g. qRT-PCR analysis of transcriptional expressions of E-cadherin in A549 cells upon MARS2 knockdown and MARS2 knockdown + KN93 (n = 9). h. qRT-PCR analysis of transcriptional expressions of E-cadherin in A549 cells upon MCU knockdown and MCU knockdown + KN93 (n = 9). i. Transcriptional expressions of MARS2, E-cadherin and Snail were evaluated by qRT-PCR with MARS2 knockdown and MARS2 knockdown + H2O2 (100 μM for 24 h) in A549 cells (n = 9). j. Effect of MARS2 knockdown on A549 cell migration was investigated by wound healing cell migration assay in A549 cells (n = 3). k. Wound-healing cell migration assay was performed with MARS2 knockdown in H2O2-treated A549 cells (n = 3). l. Activity of MMP-2 is related with MARS2 as evidenced by gelatin-zymography assay (n = 3). m. Invasive ability of H2O2-treated A549 cells was tested using Boyden chamber assay with MARS2 knockdown (n = 3). Media containing 0.1% FBS (−) were used as negative controls. All the quantitative data in graphs are marked as the mean ± S.D from at least three independent samples. Statistical analyses of results were performed with Student's t-test or ANOVA followed by Tukey's test. (*, P < 0.05, **, P < 0.01, ***, P < 0.001, ##, P < 0.01 versus si-MARS2, $$, P < 0.01 versus si-MCU).