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. 2023 Feb 6;25(3):124. doi: 10.3892/etm.2023.11823

Figure 4.

Figure 4

ABCG2 gene and protein expression in cells detected by RT-qPCR and flow cytometry. (A) The expression level of ABCG2 protein in cells was detected by flow cytometry. (B) The expression level of ABCG2 mRNA in cells was detected by RT-qPCR. The expression level of ABCG2 mRNA in HepG2/ADM and HepG2/ABCG2 cells was significantly higher than that in HepG2 and HepG2/pcDNA3.1 cells (P<0.01), and the expression level of ABCG2 mRNA in HepG2/ABCG2 cells was significantly higher than that in HepG2/ADM cells (P<0.05), but there was no significant difference between the HepG2/pcDNA3.1 and HepG2 cells. (C) The expression level of ABCG2 protein in HepG2/ADM and HepG2/ABCG2 cells was significantly higher than that in HepG2 and HepG2/pcDNA3.1 cells (P<0.01), and the expression level of ABCG2 protein in HepG2/ABCG2 cells was significantly higher than that in HepG2/ADM cells (P<0.05), but there was no significant difference between the HepG2/pcDNA3.1 and HepG2 cells. Values are expressed as the mean +/- standard deviation (n=3). **P<0.01 vs. HepG2 and HepG2/pcDNA3.1 cells; #P<0.05 vs. HepG2/ADM cells. a and b stand for ‘gates’ of flow cytometry. ADM, Adriamycin; ABCG2, adenosine triphosphate-binding cassette transporter G2; RT-qPCR, reverse transcription-quantitative PCR.