Table 2.
Flow cytometry of lymphocyte subsets in peripheral blood, serum immunoglobulins, and specific antibodies
| Patient 1* | Patient 2 | Patient 3* | Ref. values for age** | |
|---|---|---|---|---|
| WBC (cells/µL) | 7,645 | NR | 6,270 | 5,900 (4,600–7,100) |
| Lymphocytes (%) | 20 | NR | 30.2 | 32 (28–39) |
| Lymphocytes (cells/ µ L) | 1,529 | NR | 1,894 | 2,300 (1,200–4,100) |
| Lymphocyte subsets | ||||
| CD3+ (%) | 56 | NR | 71.7 | 67 (50–91) |
| CD3+(cells/µL) | 856 | 876 (700–2,100) | 1,358 | 1,500 (780–3,000) |
| CD3+/CD4+ (%) | 36.2 | NR | 44.3 | 42 (28–64) |
| CD3+/CD4+ (cells/µL) | 553 | 613 (300–1,400) | 839 | 1,000 (500–2,000) |
| CD3+/CD8+ (%) | 18.7 | NR | 23.5 | 22 (12–40) |
| CD3+/CD8+ (cells/µL) | 286 | 265 (200–900) | 445 | 500 (200–1,200) |
| CD3+/CD4+/CD8+ (%) | 0.4 | NR | 0.4 | 0.26 (0.08–0.94) |
| CD3+/CD4+/CD8+ (cells/µL) | 4 | NR | 6 | 12 (2–60) |
| CD4/CD8 ratio | 1.9 | 2.3 | 1.9 | 1.9 (1.0–3.6) |
| CD19+ (%) | 13.4 | NR | 8.5 | 10 (4–28) |
| CD19+ (cells/µL) | 205 | NR | 161 | 230 (64–820) |
| CD3−CD16+/CD56+ (%) | 24.1 | NR | 16.5 | 15 (5–49) |
| CD3-CD16+/CD56+ | 368 | NR | 312 | 340 (100–1,200) |
| CD45+/CD14+ (%) | 7.7 | NR | 5.7 | 3–8 |
| CD45+/CD14+ /µL | 591 | NR | 359 | 100–8,000 |
| Serum immunoglobulins | ||||
| IgG (mg/dL) | 1467 (540–1,822) | 1098 | ND | (814–2,047) |
| IgA (mg/dL) | 200 (63–484) | 88 | ND | (81–538) |
| IgM (mg/dL) | 213 (22–240) | 154 | ND | (42–600) |
| IgE (IU/mL) | 1,151 (0–100) | ND | ND | |
| HB IgG (mU/mL) | 0.08 (< 10) | ND | ND | |
| Rubella IgG (mU/mL) | 187 (< 10) | ND | ND | |
Values in bold are outside the normal ranges for age
ND not done, NR not reported
*Flow cytometry of lymphocyte populations performed on peripheral blood lymphocytes (PBLs) stained with fluorochrome-labeled mAbs. Cells were collected on a FACS Canto II (BD Biosciences, San José, CA), and the data were analyzed with FlowJo v8.2 (TreeStar, Ashland, OR) by gating on CD45+ leukocytes. Reference values for lymphocyte subsets from Schatorjé EJH, et al. Scand. J Immunol. 2012 vol. 75 (4) pp. 436–44
**For patient 2, only results from the BD Tritest™ (BD Biosciences) were available and reference values are as indicated in the hospital records