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. 2023 Feb 23;20:45. doi: 10.1186/s12974-023-02731-y

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© The Author(s) 2023

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — Bone marrow and blood fibrocytes in young (2.5-month) and aged (15–16-month) mice. A Gating strategies of blood fibrocytes. The gating strategies were based on relevant isotype controls. CD45⁺Collagen-1⁺ cells were considered circulating fibrocytes. B, C Percentage of CD45⁺collagen1⁺ fibrocytes in bone marrow (B) and blood (C) from young and aged mice under normal conditions. D, E Percentage of CD45⁺collagen1⁺ fibrocytes in bone marrow (D) and blood (E) from young and aged mice with/without subretinal fibrosis (SRF). Mean ± SD, n = 4–5, **p < 0.01, ***p < 0.001, Student t test. F, G Representative confocal images of blood cells stained for CD45 (green) and collagen-1 (red) from young (F) and aged mice (G) with subretinal fibrosis. Cell nuclei were stained with 4',6-diamidino-2-phenylindole (DAPI, blue), Scale bar = 20 um. (H) Magnified area of box in (G) showing CD45⁺collagen1⁺ cells (arrows) and CD45⁺collagen1⁻ cells (asterisks). Scale bar = 5 µm. I Percentage of CD45⁺collagen1⁺ fibrocytes detected by immunocytochemistry in the bone marrow and blood from young and aged mice with subretinal fibrosis. Mean ± SD, n = 3, *p < 0.05, Student t test