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. 2023 Feb 23;20:45. doi: 10.1186/s12974-023-02731-y

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© The Author(s) 2023

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — Fibrocytes in the subretinal fibrotic lesion in young and aged mice. A Representative confocal image of retinal cryosection from young (2.5-month) mice with subretinal fibrosis stained for nucleus (blue), CD45 (green) and collagen-1 (red). Cell nuclei were stained with 4',6-diamidino-2-phenylindole (DAPI, blue). (B) Magnified area of box in A showing CD45⁺collagen-1⁺ cells (arrows) in fibrotic lesion. C Representative confocal image of retinal cryosection from aged (15–16-month) mice with subretinal fibrosis stained for CD45 (green) and collagen-1 (red). (D) Magnified area of box in C showing CD45⁺collagen-1⁺ cells (arrows) in fibrotic lesion. A, C Scale bar = 50 µm; B, D Scale bar = 20 µm. E, F Number of CD45⁺ cells expressed per mm² area of lesion (E) and per lesion (F) in young and aged mice. G, H Number of CD45⁺collagen-1⁺ cells in subretinal fibrotic lesions of young and aged mice. H Percentage of CD45⁺collagen-1⁺ cells among all CD45⁺ cells in subretinal fibrotic lesions of young and aged mice. Mean ± SD, n = 17–18 lesions per group from 5 eyes, **p < 0.01, ***p < 0.001, Student t test. I, J Correlation in the number of CD45⁺collagen-1⁺ cells between retinal fibrotic lesion and bone marrow (I) or blood (J). K, L Correlation between subretinal fibrotic lesion size and CD45⁺collagen-1⁺ cells in the bone marrow (I) or blood (J)