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. 2023 Feb 23;20:45. doi: 10.1186/s12974-023-02731-y

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© The Author(s) 2023

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — Expression of fibrotic markers in bone-marrow-derived macrophages (BMDMs) from young and aged mice. Primary bone-marrow cells from young and aged mice were isolated and cultured with 20% L929 supernatant for 5–7 days to generate BMDMs for immunocytochemistry or qRT-PCR. A Representative confocal images of BMDMs stained for DAPI (blue), α-SMA (red) and collagen-1 (green) from young (2.5-month) and aged (15–16-month) mice under normal conditions. Scale bar = 100um. B Dot/bar figure showing the percentages of collagen-1⁺αSMA⁺ cells in young and aged groups. Mean ± SD, n = 4, ***p < 0.001, Student t test. C–F qRT-PCR analysis of the expression of fibrotic marker genes (Col1a1, Acta2, Fn1) and Tgfb1 in BMDMs from normal young and aged mice with or without subretinal fibrosis. C Expression of Col1a1, Acta2, and Fn1 genes in the BMDMs from normal young and aged mice. D Expression of the Tgfb1 gene in the BMDMs from normal young and aged mice. E Expression of Col1a1, Acta2, and Fn1 genes in the BMDMs from aged mice with and without subretinal fibrosis (SRF). F Expression of Col1a1, Acta2, and Fn1 genes in the BMDMs from young and aged mice with subretinal fibrosis. Mean ± SD, n = 3–4, *p < 0.05, **p < 0.01,***p < 0.001 Student t test