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. 2023 Feb 9;14:1128261. doi: 10.3389/fmicb.2023.1128261

Figure 2.

Figure 2

(A) The RFU signal generated by the PCR-LbCas12a system detecting the serially diluted standard YP_005224572.1 DNA. (B) The qPCR results of serial diluted standard DNA samples. (C) 1% agarose gel electrophoresis of the PCR product from serial diluted standard DNA samples. (D) Different pathogens was detected by PCR-Cas12a system. Only Klebsiella pneumoniae was successfully detected. Data are mean ± s. d. of n = 3 biological independent experiments. (E) Venn diagram of results of testing 89 sputum samples in disk diffusion assay and PCR-LbCas12a workflow.