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. 2023 Feb 23;18(3):636–653. doi: 10.1016/j.stemcr.2023.01.011

Figure 3.

Figure 3

Reproducibility of SARS-CoV-2 infection in airway organoids

(A) Experimental scheme of SARS-CoV-2 infection (MOI = 0.3).

(B) Representative, confocal images (z stack) of SARS-CoV-2-infected (top and middle panels) and Mock-infected (bottom panel) whole-mounted organoids. Scale bars, 50 μm. For the top and bottom, all z stacks were combined into one z stack projection throughout the entire organoid image. Middle: 3 z stacks with in-focus acTUBA staining were combined into a z-projection and deconvolved to increase contrast.

(C) Gating layouts of Spectral flow for dsRNA (cells with replicating SARS-CoV-2).

(D) % of dsRNA+ in replicate experiments of the same DDO 72 h p.i. Dots show the mean value of each experiment with three replicates.

(E) % of dsRNA + cells (left) and the bar charts (right) showing the fraction of cell types infected by SARS-CoV-2-WA-1 (dsRNA+). Bar charts show the mean value of three independent experiments with three replicates each.