P.
shigelloides |
|
|
P. shigelloides
9 |
Type
strain |
9 |
DPH-2 |
Spontaneous heme iron
utilization mutant of P. shigelloides 9 |
This study |
V. cholerae |
|
|
CA401 |
Wild type;
classical strain |
15 |
CA40130 |
Vibriobactin synthesis mutant
of CA401 |
17 |
DHH-11 |
TonB− mutant of
CA40130 |
20 |
E.
coli |
|
|
1017 |
entf::Tn5
mutant of HB101; F− Δ(gpt-proA)62 leuB6 glnV44
ara-14 galK2 lacY1 Δ(mcrC-mrr) rpsL20
Strrxyl-5 mtl-1 recA13
|
S. M. Payne |
RK1065L |
hemA |
R. Kadner |
DHE-1 |
RK1065L
entB
|
This study |
DH5α |
F−endA1 hsdR17 supE44 thi-1 recA1 gyrA96 relA1
Δ(argF-lacZYA)U169 (φ80ΔlacZ M15)
λ−
|
18 |
KP1032 |
tonB::Kan
mutant of W3110 F−
IN(rrnD-rrnE)1
|
25 |
Plasmids |
|
|
pHPS1 |
Ampr; 30-kb
(partial) Sau3A fragment of P. shigelloides 9 DNA
cloned into pJB8; carries hugAWXZ, tonB, exbBD
|
9 |
pHPS6 |
Ampr; 48-kb (partial) Sau3A
fragment of P. shigelloides 9 DNA cloned into pJB8;
hugBCD
|
P. Daskaleros |
pHUG1 |
Cmr;
pHUG2 without the 1.2-kb EcoRV-HindIII
fragment; contains P. shigelloides tonB
|
This study |
pHUG2 |
Cmr; contains 2.7-kb
AseI-HindIII fragment of pHUG10 cloned into
the EcoRV-HindIII site of pACYC184; contains
P. shigelloides tonB and exbBD
|
This study |
pHUG2.1 |
Cmr, Knr; pHUG2 with 1.2
kb-PstI fragment containing kanamycin cassette from pUC4K
cloned into PstI site of pHUG2; disrupts P.
shigelloides tonB
|
This study |
pHUG3 |
Tetr;
2.5-kb PstI fragment from pHUG10 cloned into PstI
site of pAT153; contains hugA
|
This study |
pHUG3.1 |
Cbr; 2.5-kb PstI fragment from
pHUG3 cloned into PstI site of pWSK29 |
This study |
pHUG3.2 |
Tetr; 2.5-kb PstI fragment from
pHUG3 cloned into PstI site of pLAFR3 |
This study |
pHUG4 |
Ampr; 7.5-kb
HindIII-PshAI fragment of pHUG10 cloned into
HindIII-SmaI site of pWSK29; contains
hugA, hugWXZ, tonB, exbBD
|
This study |
pHUG7 |
Ampr; 7-kb
HindIII-NheI fragment from pHUG10 cloned into
HindIII-XbaI site of pWSK29; contains
hugA and hugWXZ
|
This study |
pHUG8 |
Cmr; pHUG10 without the 2.4-kb
BsiWI fragment; contains hugA, tonbB,
exbBD
|
This study |
pHUG10 |
Cmr; 10-kb
HindIII fragment of pHPS1 cloned into pACYC184; contains
hugA, hugWXZ, tonB, exbBD
|
This study |
pHUG10.1 |
Tetr; 10-kB HindIII
fragment of pHUG10 cloned into pLAFR3 |
This study |
pHUG16 |
Tetr; 6-kB EcoRI fragment of
pHPS6 cloned into pACYC184; contains exbBD, hugBCD
|
This
study |
pHUGlac1 |
Ampr; 2.3-kb
BamHI fragment from pHUG10 cloned into pQF50; contains
putative hugAW promoter in front of lacZ
|
This
study |
pTONlac1 |
Ampr; 2.3-kb
BamHI fragment from pHUG10 cloned into pQF50 in opposite
orientation as in pHUGlac1; contains putative
tonB promoter in front of lacZ
|
This study |
pTEE1 |
Cmr; 2.1-kb PCR fragment containing V.
cholerae, tonB1, exbB1D1 cloned into pACYC184 |
38 |
pCOS3 |
Tetr; 30-kb partial Sau3A1
fragment of V. cholerae DNA cloned into pLAFR3; contains
tonB2, exbB2D2
|
38 |
pHUT3 |
Ampr; 3-kb
SalI-HindIII fragment of V.
cholerae DNA cloned into pAT153; contains hutA
|
20 |
pHUT10 |
Cmr; 10.3-kb HindIII
fragment of V. cholerae DNA cloned into pACYC184; contains
tonB1, exbB1D1, hutBCD and hutWXZ
|
20 |
pAC1 |
Cmr; pACYC184 without the
BamHI-SalI fragment containing the tetracycline
resistance gene; used to provide antibiotic resistance to DPH-2 for
triparental matings |
This study |
pETONBX |
Ampr;
PCR fragment containing E. coli tonB cloned into
pWKS30 |
A. Mey |
pACYC184 |
Cmr, Tetr;
plasmid vector |
6 |
pAT153 |
Ampr, Tetr;
plasmid vector |
56 |
pWSK29 |
Ampr; low-copy-number
plasmid vector |
59 |
pQF50 |
Ampr; plasmid vector
for construction of promoter lac fusions |
12 |
pUC4K |
Kanr, Ampr; vector containing
kanamycin cassette |
Pharmacia |
pLAFR3 |
Tetr;
mobilizable cosmid cloning vector |
49 |