TABLE 1.
Bacterial strains and plasmids
| Strain or plasmid | Description | Source or reference |
|---|---|---|
| P. shigelloides | ||
| P. shigelloides 9 | Type strain | 9 |
| DPH-2 | Spontaneous heme iron utilization mutant of P. shigelloides 9 | This study |
| V. cholerae | ||
| CA401 | Wild type; classical strain | 15 |
| CA40130 | Vibriobactin synthesis mutant of CA401 | 17 |
| DHH-11 | TonB− mutant of CA40130 | 20 |
| E. coli | ||
| 1017 | entf::Tn5 mutant of HB101; F− Δ(gpt-proA)62 leuB6 glnV44 ara-14 galK2 lacY1 Δ(mcrC-mrr) rpsL20 Strrxyl-5 mtl-1 recA13 | S. M. Payne |
| RK1065L | hemA | R. Kadner |
| DHE-1 | RK1065L entB | This study |
| DH5α | F−endA1 hsdR17 supE44 thi-1 recA1 gyrA96 relA1 Δ(argF-lacZYA)U169 (φ80ΔlacZ M15) λ− | 18 |
| KP1032 | tonB::Kan mutant of W3110 F− IN(rrnD-rrnE)1 | 25 |
| Plasmids | ||
| pHPS1 | Ampr; 30-kb (partial) Sau3A fragment of P. shigelloides 9 DNA cloned into pJB8; carries hugAWXZ, tonB, exbBD | 9 |
| pHPS6 | Ampr; 48-kb (partial) Sau3A fragment of P. shigelloides 9 DNA cloned into pJB8; hugBCD | P. Daskaleros |
| pHUG1 | Cmr; pHUG2 without the 1.2-kb EcoRV-HindIII fragment; contains P. shigelloides tonB | This study |
| pHUG2 | Cmr; contains 2.7-kb AseI-HindIII fragment of pHUG10 cloned into the EcoRV-HindIII site of pACYC184; contains P. shigelloides tonB and exbBD | This study |
| pHUG2.1 | Cmr, Knr; pHUG2 with 1.2 kb-PstI fragment containing kanamycin cassette from pUC4K cloned into PstI site of pHUG2; disrupts P. shigelloides tonB | This study |
| pHUG3 | Tetr; 2.5-kb PstI fragment from pHUG10 cloned into PstI site of pAT153; contains hugA | This study |
| pHUG3.1 | Cbr; 2.5-kb PstI fragment from pHUG3 cloned into PstI site of pWSK29 | This study |
| pHUG3.2 | Tetr; 2.5-kb PstI fragment from pHUG3 cloned into PstI site of pLAFR3 | This study |
| pHUG4 | Ampr; 7.5-kb HindIII-PshAI fragment of pHUG10 cloned into HindIII-SmaI site of pWSK29; contains hugA, hugWXZ, tonB, exbBD | This study |
| pHUG7 | Ampr; 7-kb HindIII-NheI fragment from pHUG10 cloned into HindIII-XbaI site of pWSK29; contains hugA and hugWXZ | This study |
| pHUG8 | Cmr; pHUG10 without the 2.4-kb BsiWI fragment; contains hugA, tonbB, exbBD | This study |
| pHUG10 | Cmr; 10-kb HindIII fragment of pHPS1 cloned into pACYC184; contains hugA, hugWXZ, tonB, exbBD | This study |
| pHUG10.1 | Tetr; 10-kB HindIII fragment of pHUG10 cloned into pLAFR3 | This study |
| pHUG16 | Tetr; 6-kB EcoRI fragment of pHPS6 cloned into pACYC184; contains exbBD, hugBCD | This study |
| pHUGlac1 | Ampr; 2.3-kb BamHI fragment from pHUG10 cloned into pQF50; contains putative hugAW promoter in front of lacZ | This study |
| pTONlac1 | Ampr; 2.3-kb BamHI fragment from pHUG10 cloned into pQF50 in opposite orientation as in pHUGlac1; contains putative tonB promoter in front of lacZ | This study |
| pTEE1 | Cmr; 2.1-kb PCR fragment containing V. cholerae, tonB1, exbB1D1 cloned into pACYC184 | 38 |
| pCOS3 | Tetr; 30-kb partial Sau3A1 fragment of V. cholerae DNA cloned into pLAFR3; contains tonB2, exbB2D2 | 38 |
| pHUT3 | Ampr; 3-kb SalI-HindIII fragment of V. cholerae DNA cloned into pAT153; contains hutA | 20 |
| pHUT10 | Cmr; 10.3-kb HindIII fragment of V. cholerae DNA cloned into pACYC184; contains tonB1, exbB1D1, hutBCD and hutWXZ | 20 |
| pAC1 | Cmr; pACYC184 without the BamHI-SalI fragment containing the tetracycline resistance gene; used to provide antibiotic resistance to DPH-2 for triparental matings | This study |
| pETONBX | Ampr; PCR fragment containing E. coli tonB cloned into pWKS30 | A. Mey |
| pACYC184 | Cmr, Tetr; plasmid vector | 6 |
| pAT153 | Ampr, Tetr; plasmid vector | 56 |
| pWSK29 | Ampr; low-copy-number plasmid vector | 59 |
| pQF50 | Ampr; plasmid vector for construction of promoter lac fusions | 12 |
| pUC4K | Kanr, Ampr; vector containing kanamycin cassette | Pharmacia |
| pLAFR3 | Tetr; mobilizable cosmid cloning vector | 49 |