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P. shigelloides HugA functions as a heme receptor in E. coli
| E. coli 1017 transformed with plasmid: | Absorbance of
cultures grown ina:
|
||
|---|---|---|---|
| L broth | L broth with EDDA | L broth with EDDA plus heme | |
| pHUT10/pHUT3 (V. cholerae system) | 1.20 | 0.05 | 0.95 |
| pHUT10/pHUG3 (hybrid system with P. shigelloides hugA) | 1.20 | 0.06 | 1.25 |
| pHUT10 (no receptor gene) | 1.02 | 0.05 | 0.08 |
Absorbance at 650 nm was taken after 18 h of growth.
