TABLE 4.
Characterization of a P. shigelloides heme utilization mutant
| P. shigelloides strain | Growth with the iron
sourcea:
|
||
|---|---|---|---|
| Heme | Hemoglobin | FeSO4 | |
| P. shigelloides 9 (wild type) | +b | + | + |
| DPH-2 (heme utilization mutant) | −c | − | + |
| DPH-2/pHUG10.1 (contains hugAWXZ, tonB, exbBD) | + | + | + |
| DPH-2/pHUG3.2 (contains hugA) | + | + | + |
| DPH-2/pHUT2 (contains V. cholerae hutA) | + | + | + |
| DPH-2/pHUT4 (contains V. cholerae tonB1, exbB1D1, hutBCD, hutWXZ) | − | − | + |
a
Strains were seeded into L agar containing 50 μg of EDDA per ml at 105 cells/ml. Five microliters of 40 μM heme, 10 μM hemoglobin, or 10 mM FeSO4 was spotted onto the plates, and the plates were incubated for 15 h.
b
A plus represents a zone of growth of at least 8 mm around the spot of FeSO4 and 10 mm around the spots of heme and hemoglobin.
c
A minus indicates no detectable growth.