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Complementation of an E. coli tonB mutant with the P. shigelloides TonB system genes
| E. coli KP1032 transformed with plasmid: | Absorbance of cultures grown
ina:
|
|
|---|---|---|
| L broth | L broth with EDDA (enterobactin utilization) | |
| No plasmid (TonB−) | 1.49 | 0.23 |
| pETONBX (E. coli tonB) | 1.48 | 1.51 |
| pHUG2 (P. shigelloides TonB system) | 1.55 | 1.34 |
| pHUG1 (P. shigelloides tonB) | 1.50 | 0.03 |
Absorbance was taken at 600 nm after 17 h of growth.
