TABLE 6.
E. coli 1017 transformed with plasmid: | Growth in media
containinga:
|
||||
---|---|---|---|---|---|
L broth
|
L broth with EDDA (low salt) | L broth with
EDDA plus heme
|
|||
Low salt | High salt | Low salt | High salt | ||
pHUG7/pHUG2 (P. shigelloides TonB) | 1.94 | 1.56 | 0.02 | 1.77 | 1.37 |
pHUG7/pTEE1 (V. cholerae TonB1) | 2.20 | 1.77 | 0.00 | 1.90 | 1.40 |
pHUG7/pCos3 (V. cholerae TonB2) | 2.03 | 1.76 | 0.05 | 1.30 | 0.40 |
Strains were grown in L broth containing 170 mM NaCl (low salt) or 625 mM NaCl (high salt) containing no EDDA or containing 200 μg of EDDA per ml. with or without hemin. Absorbance at 600 nm was taken after 18 h of growth.