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. 2023 Feb 9;10:1114726. doi: 10.3389/fcvm.2023.1114726

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Copyright © 2023 Thompson, Janda and Schnellmann.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Matrigel embedded capillary sprouting capacity and Griffonia Simplicifolia Lectin-IB4 IF-staining of isolated MRPECs 10 days post-isolation. Representative phase-contrast photomicrographs and IF-images of MRPECs embedded in a Matrigel assays 36 h after Griffonia Simplicifolia Lectin-IB4 (GSL-1) staining and implantation. (A) Nuclear staining, DAPI (blue), (B) GSL-I staining (green), (C) phase-contrasted photomicrograph of embedded MRPECs (gray), and (D) overlay (merged); white arrows indicate capillary spouts/tubes. All representative images were taken at 20× magnification. All images were obtained on an EVOS-M5000 fluorescence microscope system and merged by overlay in FIJI (ImageJ) software. N = 4 isolations. Scale bars, 50 μm.