TABLE 1.
Synthesis of Amblyomin-X domains and related peptides used in the study. Amino acid sequence and Molecular weight (average) by ESI mass spectrometry. *: amidation; C#: the 2 cysteines are connected by a disulfide bond.
| Compounds | Amino acid sequence | MW average found | MW average calculated |
|---|---|---|---|
| Kunitz: 1-57 (57AA) | ANSKAVCNLPKAGDETCSNKTEIRWYYNGTACEAFIFKGCGGNDNNFDRVDDCQRLC-NH2 | 6434.1±0.1 | 6434.16 |
| C-ter: 58-106 (49AA) | EEQTHFHFESPKLIFKVQDYWILNDIMKKNLTGISLKSEEEDADSGEID | 5856.2±0.1 | 5856.4 |
| TAT-Kunitz: 1-71 (71AA) | GRKKRRQRRRPPQANSKAVCNLPKLAGDETCSNKTEIRWYYNGTACEAFIFKGCGGNDNNFDRVDDCQRLC-NH2 | 8134.8±0.3 | 8135.17 |
| p15-Cter: 1-61 (61AA) | C#WMSPRHLGT C#EEQTHFHFESPKLISFKVQDYWILNDIMKKNLTGISLKSEEEDADSGEID | 7127.0±0.1 | 7126.9 |
| TAT:1-13 (13AA) | GRKKRRQRRRPPQ | 1718.01 | 1718.03 |
| p15:1 -11(11AA) | C#WMSPRHLGTC# | 1287.4 | 1287.5 |
| F1C: 1-20 (20AA) | EEQTHFHFESPKLISFKVQD* | 2445.6 | 2445.7 |
| F2C: 16-35 (20AA) | FKVQDYWILNDIMKKNLTGI* | 2438.8 | 2438.9 |
| F3C: 31-50 (20AA) | NLTGISLKSEEEDADSGEID | 2122.16 | 2122.18 |