TABLE 4.

Cellular-uptake efficiency low of Kunitz molecule. Tumor cells were treated with Alexa-Fluor 488-labeled molecules at indicated times. Fluorescence were monitored by a Molecular Devices ImageXpress Micro Confocal plate-scanning microscope (Molecular Devices, Sunnyvale, CA). Vesicles counts were analyzed using MetaXpress software (Molecular Devices, CA) with Transfluor Application Module.

Cell type	Time (h)	Kunitz (10 µM)	Kunitz (100 µM)	Amblyomin-X (20 µM)
SK-MEL-28	2	0.26 $\pm$ 0.024	4.14 $\pm$ 3.76	139.57 $\pm$ 71.44
	4	1.12 $\pm$ 0.11	37.64 $\pm$ 0.16	988.76 $\pm$ 409.20
MIA PaCa-2	2	0.8 $\pm$ 0.28	13 $\pm$ 5.93	76.69 $\pm$ 20.72
	4	1.86 $\pm$ 1.31	98 $\pm$ 49.73	1672.38 $\pm$ 395.60