FIG 5.

Conjugation rates and tra gene expression levels for evolved MG1655 plasmid carriers. Dots with no outline show conjugation rate estimates for evolved MG1655 clones facteted per replicate line evolved with (blue) or without (red) cefotaxime selection; data are shown for 3 technical replicates of the conjugation assay; the solid black line shows the ancestral conjugation rate and the dotted lines ± standard error. Dots with black outline show mean conjugation rates for evolved MG1655 clones relative to their ancestor plotted against the corresponding mean fold change in tra gene expression relative to their ancestor for the same evolved clone. The blue line is a linear regression and the gray ribbon indicates ± standard error.