FIG 6.
Transcriptional and resistance effects of IS-element insertions upstream of hns. Orientation and location of IS-element insertions between hns and tdk in 3 E. coli MG1655 plasmid-carrier evolved clones. (A) The reference configuration of the region, and the insertion position and orientation of IS-elements in evolved clones. (B) Differential expression of hns, tdk and blaCTX-M-15 for all MG1655 plasmid-carrier evolved clones relative to their ancestor. Red cells indicate increased expression, blue cells indicate decreased expression relative to their ancestor. Darker shading indicates fold change Log2 FC ≥1.0 relative to the clones lacking an IS-element insertion upstream of hns. (C) MIC curves against cefotaxime showing variation between the individual evolved and ancestral clones for the EP and EX treatments (different evolving lines denoted by colors; see visual key). (D) normalized transcript counts of blaCTX-M-15 (counts per million) with the different evolving lines denoted by colors (see visual key).