TABLE 9.

PERMANOVA analysis of the effect of 1 g berberine/kg feed supplementation on chicken gut microbiota molecular functions dissimilarities based on Bray-Curtis^a

Functional microbiota	Bray-Curtis
	R 2	P value
Day 12
Jejunum	1.8%	0.648
Ileum	7.9%	0.068
Cecum	6.9%	0.110
Colon	10.7%	0.085
Day 21
Jejunum	11.1%	0.042
Ileum	9.2%	0.108
Cecum	27.4%	0.006
Colon	31.9%	0.007

^aP values were calculated on 999 possible permutations. Bold values indicate statistical significance (P value < 0.05). R² represents the percentage of variation accountable for the supplementation of berberine in the feed.