Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Feb 8;220(4):e20221288. doi: 10.1084/jem.20221288

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

This is a work of the U.S. Government and is not subject to copyright protection in the United States. Foreign copyrights may apply.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Figure 4. — Ablating CTCF impairs T_EFF cell accumulation and cytotoxic functions. (A) Immunoblotting for CTCF in sorted WT or Ctcf^−/− GFP⁺ naive CD8⁺ T cells (before adoptive transfer, left) and early T_EFF cells (4 dpi from infected recipients), with β-actin as loading control. Data are representative from two independent experiments. (B and C) Cell division of CTV-labeled WT or Ctcf^−/− naive CD45.2⁺GFP⁺CD8⁺ T cells at 36 h (top) or 60 h (bottom) after transfer into CD45.1⁺ recipient mice followed by LCMV-Arm infection, with representative dot plots (B) and cumulative data on cell frequency in each cell division (C) shown. (D and E) Relative expression of CD25 (D) and CD69 (E) in WT or Ctcf^−/− CD8⁺ T cells in individual cell divisions at 36 or 60 h after infection. gMFI, geometric mean fluorescence intensity. (F) Numbers of WT or Ctcf^−/− effector CD8⁺ T cells on 4 and 8 dpi. (G–I) Detection of CD25 expression (G), IFN-γ production (H), and granzyme B expression (I) in WT or Ctcf^−/− CD8⁺ T cells on 4 dpi. Data in B, H, and I are from two independent experiments. Data in C–F are means ± SD. *, P < 0.05; **, P < 0.01; ***, P < 0.001; NS, not statistically significant by two-tailed Student’s t test. Source data are available for this figure: SourceData F4.