Figure S5.

T_MP genes are harbored in insulated neighborhoods demarcated by constitutive CTCF binding and gain access to Tcf1 in CTCF-deficient T_EFF cells. (A) Tracks of CTCF CUT&RUN, ATAC-seq, and Tcf1 ChIP-seq at the Il7r and Sell gene loci linked to the incongruous sites (denoted with blue bars). The green bars denote ChrAcc⁻ constitutive CTCF binding sites that are positioned at the boundaries of insulated neighborhoods. (B) Diagram illustrating the relative positions of Hi-C–based ChrInt pile-up profile centering at sites of interest. The incongruous site-centered heatmap (from Fig. 8 A, without mirroring image) is in the middle, and flanked by the boundary anchor–centered heatmaps (from Fig. 8 B, without mirroring image) on each side. Summation of interactions between the boundary anchors (from Fig. 8 C) is positioned on the top, as insulating knots. (C) ChrInt changes between T_EFF and T_N cells in the insulated neighborhoods harboring Tcf7, Sell, and Id3 genes. The insulated neighborhoods are marked in triangles in solid green lines in Fig. 8 E, and changes in each ChrInt pair within the neighborhoods between T_EFF and T_N cells are summarized in boxplots, where center lines denote the median, box edge denotes IQR, and whiskers denote the most extreme data points that are no more than 1.5 × IQR from the edge. (D) PCA of Tcf1 binding profile in WT T_N, WT and Ctcf^−/− T_EFF cells, where library size-normalized Tcf1 CUT&RUN signals on merged peaks were used as input data.