Table 1.
Mapping rate and coverage uniformity of Olivar Sars-Cov-2 primers and ARTIC v4.1 primers.
| sample concentration1 | mapping rate2 | less than 0.05 × median coverage3 | 0.1 × to 10 × median coverage | |||||
|---|---|---|---|---|---|---|---|---|
| Olivar | ARTIC | Olivar | ARTIC | Olivar | ARTIC | |||
| synthetic RNA control | Ct=18 (~ 5 × 105 copy/ul) | replicate 1 | 86.7% | 88.1% | 3.2% | 5.9% | 92.5% | 93.1% |
| replicate 2 | 90.2% | 85.9% | 3.7% | 6.0% | 92.7% | 91.5% | ||
| Ct=35 (~ 3 copy/ul) | replicate 1 | 92.6% | 89.4% | 10.1% | 18.5% | 73.4% | 71.5% | |
| replicate 2 | 88.8% | 84.5% | 10.7% | 20.6% | 69.8% | 73.3% | ||
| wastewater site: CB | 106.9 copy/ul | Aug. 08, 2022 | 15.0% | 4.6% | 10.4% | 14.1% | 65.6% | 70.5% |
| 83.9 copy/ul | Aug. 15, 2022 | 52.7% | 42.9% | 8.8% | 15.6% | 77.8% | 71.9% | |
| wastewater site: KB | 28.1 copy/ul | Aug. 08, 2022 | 22.0% | 10.6% | 19.1% | 17.3% | 64.1% | 59.3% |
| 35.4 copy/ul | Aug. 15, 2022 | 27.6% | 20.0% | 12.4% | 11.6% | 72.4% | 64.2% | |
1
Concentration of wastewater samples are measured with ddPCR.
2
Percentage of concordant read pairs mapped to the reference. Average of pool 1 and pool 2.
3
Percentage of bases with the corresponding coverage.