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[Preprint]. 2023 Feb 15:2023.02.14.528512. [Version 1] doi: 10.1101/2023.02.14.528512

Fig. 4: H3K27me3 patterns in aging are mimicked by deep quiescence cultures (hyper-quiescence).

Fig. 4:

(A) RT-qPCR analysis of Ezh1, Ezh2, Kdm6a and Kdm6b RNA levels in young and old livers. Data are summarized as mean ± S.E.M. (n=8 biological replicates per group). (B) Western blot of H3K27me3, H3, EZH1, EZH2, KDM6A and KDM6B protein levels in young and old livers. (n=3 biological replicates per group). (C) Western blot of H3K27me3, H3, EZH1 and EZH2 in proliferating and quiescent cells over 14 days. Quiescence was induced by contact inhibition (n=2 biological replicates per group). (D) Heatmap showing EZH2 signal at differential peaks from Fig. 1G. (E) Genome browser snapshots of EZH2 enrichment over 4 peak regions from (D). (F) Western blot of H3K27me3, H3, EZH1 and EZH2 from different salt fractions of chromatin from Fig. 2B in young and old livers. On the right is the Ponceau S-stained membrane from the same experiment.