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[Preprint]. 2023 Aug 25:2023.02.13.528384. Originally published 2023 Feb 14. [Version 2] doi: 10.1101/2023.02.13.528384

Fig. 3: Alanine transaminase inhibition reduced HSC activation.

Fig. 3:

a, Schematic for the reaction catalyzed by ALT2. COL1A1, COL1A2, and COL3A1 gene expression in LX2 cells treated with/without TGFβ (5 ng/mL), glutamine (2 mM), and β-chloroalanine (BCLA; 0.05, 0.5, 1 mM). b, Western blot images for COL3, COL1A1, PAI1, HIF1α, and α-Tubulin in LX2 cells treated with BCLA. c, Protein abundance and gene expression of activation markers in either GPT2 knockdown LX2 or GPT2/MPC2 knockdown LX2 cells. For protein abundance, all groups were treated with 5 ng/mL TGFβ-1. d, Western blot images for COL1A1, HIF1α, and α-Tubulin in LX2 cells treated with or without glutamine (Gln, 2 mM) or dm-αKG (5 mM) compared with TGFβ-1 (5 ng/mL) treated cells.