TABLE 1.
Bacterial strains, phage, and plasmids
| Strain, phage, or plasmid | Relevant genotype | Reference or source |
|---|---|---|
| Strains | ||
| W3110 | F−; prototroph | |
| W3350 | F−gal-1 gal-2 | |
| MC4100 | F− Δ(argF-lac)U169 araD139 rpsL150 relA1 thiA deoC1 ptsF25 flbB5301 | 7 |
| Q13 | Hfr met ma pnp tyr | 33 |
| KY1461 | F− Δ(ara-leu)697 Δ(lac-pro) thi trpA38(Oc) | 25 |
| MG1655 | F−; prototroph | 12 |
| UM122 | Hfr thi katF::tet | 22 |
| ME8465 (JB100) | Hfr mal-1(Tc) ompR::tet | 5 |
| CU284 | MC4100 Δhns::kan | 48 |
| YK4207 | YK1100 Δ3[hip]::cat Δ82[himA]::tet trp+ | 11 |
| YK1100 | W3110 trpC9941 | C. Yanofsky |
| AQ7996 | fis::kan | 4 |
| KI251 | F−cya-283 ilv::tet trpR trpA9605 his-29 | 1 |
| CF1678 | MG1655 ΔrelA251::kam ΔspoT209::cat | 47 |
| KY1471 | KY1461 katF::tet | This work |
| KY1472 | KY1461 ompR::tet | This work |
| KY1473 | KY1461 Δhns::kan | This work |
| KY1474 | KY1461 Δ3[hip]::cat Δ82[himA]::tet | This work |
| KY1475 | KY1461 fis::kan | This work |
| KY1476 | KY1461 cya-283 | This work |
| Phage | ||
| λ pF13 | imm21 | 15 |
| λ pF13(Psra-lacZ) | imm21 Psra-lacZ | This work |
| Plasmids | ||
| pKV7350 | pUC119-277L | This work |
| pKV7351 | pUC119-277L sra::kan | This work |
| pUC-4K | ||
| pBAD-αβγ | 51 | |
| pMS4342 | 15 | |
| pFF6 | 18 | |
| pMS4342(Psra-lacZ) | This work | |
| pFF6(Psra-lacZ) | This work |