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[Preprint]. 2023 Feb 22:2023.02.16.528900. [Version 2] doi: 10.1101/2023.02.16.528900

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Figure 4. — Endogenous tagging reveals flagellar localization of candidate flagellar proteins: calpain 1.3-smFLAG (TcCLB.506563.200), CARP3-smFLAG (TcCLB.506681.40), or hypothetical protein-FLAG (TcCLB.510329.180) in T. cruzi epimastigotes (A) or intracellular amastigotes (B). In all cases, the FLAG tag was detected in fixed parasites using an anti-FLAG antibody and secondary antibody (green) and the flagellum was detected using anti-FCaBP and secondary antibody (magenta). The FLAG signal in the flagellum is indicated (yellow arrow).