Figure 3:

Cinnamein inhibits LPS- and polyIC-induced production of TNFα in primary mouse microglia.

Microglia isolated from 2d old mouse pups were incubated with different concentrations of cinnamein for 6 h followed by stimulation with either 1 μg/ml LPS (A) or 50 μg/ml polyIC (B) under serum-free condition. After 24 h of stimulation, the level of TNFα was measured in supernatants by ELISA. Results are mean + SD of three independent experiments. *p < 0.05; **p < 0.01; ***p < 0.001; NS, not significant.