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. Author manuscript; available in PMC: 2023 Feb 23.
Published in final edited form as: Nat Struct Mol Biol. 2022 May 9;29(5):451–462. doi: 10.1038/s41594-022-00764-0

Extended Data Fig. 5. Synchronization of ICL unhooking by NEIL3-depletion and add back.

Extended Data Fig. 5

a, NEIL3 and HMCES immunodepletion. The extracts used in the replication reactions shown in Fig. 3b were blotted for HMCES and NEIL3. Asterisks, non-specific bands.

b, pICLAP was replicated in the presence of [α−32P]dATP in the indicated extracts (shown in a) supplemented with p97 inhibitor for 60 min. rNEIL3 was then added to the reactions to allow ICL unhooking. Replication intermediates were analyzed as in Fig. 2a.

c, HMCES immunodepletion. The extracts used in Fig. 4 were blotted for HMCES. Asterisk, non-specific band.