Extended Data Fig. 10. Efficacy of Selinexor combined with AKT inhibition on leukemia initiating cells.
a) Leukemic burden of mice (n=3 biologically independent mice per group) prior to sorting and engraftment for extreme limiting dilution assay. P-values calculated using unpaired two-sided Welch’s test; data are presented as mean +/−s .d.
b) FACS plots depicting gating strategy to isolate dsRed+ MLL-AF9 cells prior to engraftment for extreme limiting-dilution assay.
c) Kaplan-Meier curves showing overall survival of secondary recipient mice (n=5 biologically independent mice) from each group in limiting dilution assay for determining LIC frequency. Statistical significance determined by log-rank (Mantel-Cox) test. n.s, not significant.