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. Author manuscript; available in PMC: 2023 Feb 23.
Published in final edited form as: Nat Cancer. 2022 Jun 6;3(7):837–851. doi: 10.1038/s43018-022-00394-x

Extended Data Fig. 3. Gating strategy and Gene Ontology analysis of FACS-based CRISPR/Cas9 screen.

Extended Data Fig. 3

a) Scatterplot depicting gating strategy to isolate live sgRNA library transduced OCI-AML2 cells based on forward-scatter (FSC) and side-scatter (SSC).

b) Scatterplot depicting gating strategy to isolate singlet sgRNA library transduced OCI-AML2 cells based on FSC.

c) Gene ontology (GO) analysis table of scoring genes enriched in the bottom sort (FSGS of > 1.5). P-values calculated by Enrichr using Benjamini-Hochberg correction for multiple hypothesis testing.