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Primer extension mapping of the 5′ end of the pyrRPB-carA mRNA. Sequencing ladders generated with the oligonucleotide used for the primer extension were loaded next to the reaction mixture. The DNA sequence of the sense strand around the first nucleotide in the transcript (+1) is presented, and the −10 sequence is boxed. The experiment was conducted on RNA extracted from cells growing exponentially in the presence (+) and absence (−) of uracil at 200 μg/ml.
