TABLE 2.

Synthetic oligonucleotides used in PCR amplifications

Primer	Sequence (5′–3′)	Usagea	Coordinateb
PyrR_Ctny	AAACTGCAGCTTAATCTTCACGTTTGATT	Cloning	945
PyrR_F4	CCTTTAAGATTGTCCAGAGAG	RT-PCR	248
PyrR_F5	GACGGACATCTTATTAATGG	RT-PCR	369
PyrRPintR	GTTGAGCCAAACATGG	RT-PCR	1147
CarA-R6	GCTCTTGTATCTACACCA	RT-PCR	3952
PyrR-Nterm	AGCTCGAGAATGAAAGGAGCCCC	Cloning	440
PyrB-Nterm	AGCTCGAGGGTAAAGGAGACCTT	Cloning	2603
PyrB-Cterm	AAACTGCAGCTTACTTCGCTTTTTTTCC	Cloning	3518
PyrP_F2	GACAGGGGATGGTTTAG	RT-PCR	1927
PyrR_4F	CAACTTAGAGTTTTAGGAG	Cloning	1057
PyrR_2R	CGGAATGGACGTGTATCC	EGW	639
PyrR_3R	AGTGATCCGTGTAATTGC	Primer ext., EGW, RT-PCR	484
PyrR_4R	CCAAAGAGAATGCAGGTAG	EGW, RT-PCR	309
PyrB_14R	CTCCACCAATAACTGGTG	Cloning	2101
PyrRB_3F	GGATACACGTCCATTCCG	RT-PCR	657
pyrB2	CATATGCTCTTACGCGTG	RT-PCR	3278
pyrB_12R	CACCTTTACTGATTGAACTGGC	RT-PCR	2846
PyrRB_2F	GCATTCTCTTTGGAATGCAGC	RT-PCR	336
Prbind-1	TCCAGAGAGGCTNGCAAG	PCR	257
PyrB11	CACGCGTAAGAGCATATG	PCR	3260
EcoRI	NNNNNNNNNNGAATTC	EGW
HindIII	NNNNNNNNNNAAGCTT	EGW
Sau3AI	NNNNNNNNNNGATC	EGW

^aThe applications of the different primers used in this study. Cloning, obtaining L. lactis DNA used for construction of plasmids; RT-PCR, primers used in the RT-PCR experiments; EGW and PCR, primers used for obtaining DNA templates for sequencing; primer ext., primer used in the primer extension experiment. 

^bThe coordinates refer to the corresponding 3′ end of pyrRPB-carA database sequence AJ13262.