FIG. 2.
Molecular weight determination of expressed KinA domains by gel filtration. The molecular weights of expressed KinA fragments were analyzed using a Sephacryl S-100 FPLC column. Standard molecular weight markers (⧫) were bovine serum albumin (66 kDa), carbonic anhydrase (29 kDa), cytochrome c (12.4 kDA), and aprotinin (6.5 kDa). The column's voided volume (V0) was determined by the migration of dextran blue (2,000 kDa). KinA-N, KinA-M, and KinA-C were eluted in a single peak at the elution volumes (Ve) of 66, 28, and 41 ml, respectively. Their molecular sizes were determined as 13, 60, and 73.3 kDa.