FIG. 4.

Phosphorylation activity of KinA and KinA-C. Various combinations of KinA (0.5 μM), KinA-C (5 μM), and Spo0F (10 μM), as indicated, were incubated with [γ-³²P]ATP at 25°C for either 3 min or 1 h, as indicated. The phosphorylated proteins were subjected to SDS-PAGE and autoradiography as described in Materials and Methods.