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Phosphotransfer reactions of KinA, KinA-C, and NRII. Various combinations of KinA (0.5 μM), KinA-C (5 μM), Spo0F (10 μM), NRII (0.5 μM), and NRI (5 μM) were incubated with [γ-32P]ATP at 25°C for 3 min. Individual reaction contents are indicated above the gel.
