FIG. 7.

Phosphotransfer from KinA∼P and KinA-C∼P to ADP. Purified γ-³²P-labeled KinA∼P (10 pmol) or KinA-C∼P (10 pmol) was incubated in the presence or absence of ADP (400 μM) as indicated in reaction buffer at 25°C for 3 min. The samples were subjected to TLC and autoradiography as described in Materials and Methods.