Table 3.

Genes of interest with direct roles in nectary formation with relative expression values from our transcriptomic dataset, putative roles and relevant citations.

Gene	SDE	Putative Role	Citation
AP3	↑S3 vs S1/S2	Downregulation disrupts nectary placement and nectar secretion in Arabidopsis	Baum et al., 2001
PI	↓S1 vs S3	Downregulation disrupts nectary placement and nectar secretion in Arabidopsis	Baum et al., 2001
AP2	≈	Downregulation disrupts nectar secretion in Arabidopsis	Baum et al., 2001
AG	↓S1 vs S2/S3	Redundantly activates CRC with SHP1/2	Morel et al., 2018
ARF8	↓S1 vs S2/S3	Affects nectary size and gene expression redundantly with AUXIN RESPONSE FACTOR 6 (ARF6) in Arabidopsis	Reeves et al., 2012
ARF6	↓S1 vs S2/S3	Affects nectary size and gene expression redundantly with ARF8 in Arabidopsis	Reeves et al., 2012
BOP2	≈	Promotes the formation of nectary glands independent of CRC	Mckim et al., 2008
SHP1/2	↑S2 vs S3	Redundantly regulates CRC with AG	Morel et al., 2018
STY	↓S1 vs S2/S3	Controls nectary development in Aquilegia independent of CRC	Min et al., 2019
CRC	≈	Essential but not sufficient for nectary formation in the core eudicots.	Lee et al., 2005b

SDE, Significant Differential Expression with arrows representing either up or down regulation of expression between developmental stages (≈ indicates no SDE). S1, pre-anthetic nectary; S2, anthetic nectary; S3, post-anthetic nectary.