Figure 5: Conserved function of ARID1A in human T cells in vitro and in vivo.

(A) Proliferation and viability of primary human T cells after electroporation of the indicated RNP. Left: Acutely stimulated T cells. Right: Chronically stimulated T cells using anti-CD3-coated plates. Data shown is representative of 3 independent experiments and 3 donors. Error bars denote mean ± SD and significance was assessed by Student’s t-test, n=2 replicates per sgRNA. (B) Schematic of CRISPR mini-pool screen in primary human CD8⁺ T cells transduced with the NY-ESO-1-specific TCR, 1G4. (C) Results of the human CRISPR mini-pool screen aggregated by gene. (D) Results of the human CRISPR mini-pool screen with individual sgRNA replicates shown as dots. Genes are ordered from highest to lowest average LFC. Box plots show 25^th, 50^th (median), and 75^th percentiles. Results shown in (C-D) are combined from 2 independent donors, 2 mice per donor, and 2 sgRNAs per target gene (n=8 sgRNA replicates per target). In (C-D), orange indicates inhibitory receptors, red indicates TCR signaling pathway genes, blue indicates chromatin remodelers and grey indicates negative controls. See also Table S5.