Figure 1. CD34-dependent micro vessel density (MVD) in periapical cyst tissues A) A PC case presenting CD34 over-expression and increased MVD. Note the brown continuous strong staining pattern (>15) in an endothelial ring like vessels B) Implementation of a DIA assay for estimating MVD rates with CD34 over-expression. The process is divided in three stages (1-3): first digitized snapshot image phase; second, marking phase using a digital red paint; and third, a measurement phase with red/green vessels’ encircling and calculating MVDs in the examined cases (original magnification 400×, MVD, DAB brown chromogen for immunohistochemistry protocol).