TABLE 3.
Effects of different transposon insertions in nreB on nickel resistance of E. coli strain S17-1
| Plasmid, Tn5 insertiona (nucleotide no.) |
MICb of Ni2+ (mM) |
|---|---|
| pVDZ'2 | 0.3 |
| pVDZ'2::TEC9 | 5 |
| pVDZ'2::TEC9, Tn5-1 (2397) | 3 |
| pVDZ'2::TEC9, Tn5-2 (2535) | 0.3 |
| pVDZ'2::TEC9, Tn5-3 (2876) | 0.3 |
| pVDZ'2::TEC9, Tn5-4 (3239) | 0.3 |
| pVDZ'2::TEC9, Tn5-5 (3573) | 0.3 |
| pVDZ'2::TEC9, Tn5-6 (3703) | 3 |
| pVDZ'2::TEC9, Tn5-7 (3718) | 3 |
a
Tn5 insertion loci were determined by DNA sequencing. The nucleotide numbers shown correspond to the sequence with accession no. L31491.
b
The MIC was defined as the concentration at which no growth was observed. Cells were grown overnight, resuspended in saline (0.85%, wt/vol), and streaked on TES agar with NiCl2 at 0, 0.3, 0.5, 1.5, 3, 5, or 7 mM, and growth was monitored at 37°C.