Extended Data Figure 13|. Dysbalance between myHSC and cyHSC occurs in advanced liver disease and elevates HCC risk in patients.
a, UMAP visualization showing that the genes from the myHSC and cyHSC signatures, encoding secreted mediators, are strongly enriched in myHSC or cyHSC, and weakly or not expressed in other liver cell populations in 8xCCl4 injured mouse liver (n=3 mice) analysed by scRNAseq. b, myHSC and cyHSC secreted gene signatures were applied to bulk RNA-seq data from cohorts of patients with HCV-induced liver disease (GSE10140) or NAFLD/NASH-induced liver disease (GSE49541), and the proportion of patients with high cyHSC/myHSC dysbalance was determined as described in the methods. c, HCC incidence was compared between patients with high cyHSC/myHSC dysbalance (i.e. a status with higher myHSC and lower cyHSC) and low cyHSC/myHSC dysbalance (i.e. a status lower myHSC and higher cyHSC) in a HCV-induced liver disease cohort (GSE15654). Statistics: data in b were analysed by two-sided Fisher’s exact test. Survival curves in c were represented using the Kaplan-Meier method and compared with log-rank statistics.