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. 2022 Nov 14;30(2):341–355. doi: 10.1038/s41418-022-01087-9

Fig. 3. SNORD88C promotes NSCLC migration and invasion in vitro and in vivo.

Fig. 3

A, B Wound healing, migration and invasion assays showed that knockdown of SNORD88C in A549 cells inhibited the motility, migration and invasion of NSCLC, while overexpression of SNORD88C in H1299 cells showed the opposite effect. Original magnification for wound healing, ×40; Original magnification for migration and invasion, ×200. C Western blot showed that SNORD88C knockdown decreased the expression of N-cadherin, Vimentin, and Snail and increased E-cadherin expression in A549 and H1299, while overexpression of SNORD88C showed the opposite expression for these proteins in SPC-A1 and H1299 cells. D, E In A549 pulmonary metastasis model the luciferase signal intensities in the LV-sh-SNORD88C group was remarkably lower than that in the control. F H&E staining showed that metastatic nodules in the lung tissue invaded the pulmonary capsule in the LV-sh-NC group, but not in the LV-sh-SNORD88C. Scale bar indicates 200 μm; Two-tailed unpaired t-test, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.