FIG. 2.

In vivo effects of IHF on transcription from the Po promoter. (A) Luciferase activity of E. coli S90C (closed squares) and its IHF⁻ counterpart, DPB101 (open squares), harboring the reporter plasmid pVI466 (dmpR-Po-luxAB). (B, C, D and E) Luciferase activity of P. putida KT2440::dmpR (closed symbols) and its IHF counterpart, KT2440-ΔihfA::dmpR (open symbols), harboring the Po-luxAB reporter plasmids pVI360 (circles), pVI590 (diamonds), pVI363 (up triangles), and pVI591 (down triangles), respectively. Schematic inserts indicate the number and derivation of the UASs on the different reporter plasmids. The results are representative of duplicate independent experiments.