FIG. 4.

In vivo effects of the relative phasing of binding motifs on transcription from the Po promoter. The luciferase activity of P. putida KT2440::dmpR harboring reporter plasmid pVI580 and derivatives pVI581 to pVI584 harboring inserts between UAS1 and UAS2 (A) or derivatives pVI585 to pVI589 harboring inserts between UASs and Po (B) and the levels obtained with the indicated derivatives in isogenic IHF⁺ and IHF⁻ P. putida strains as described in the legend to Fig. 2 (C). Values are the averages of peak activities from two independent experiments where the luciferase activity was followed over the entire growth curve. Error bars, standard errors.